中國農業科學 ?? 2008, Vol. 41 ?? Issue (6): 1872-1878 .doi: 10.3864/j.issn.0578-1752.2008.06.040

? 研究簡報 ? 上一篇    

中國荷斯坦牛STAT5A基因遺傳多態性與泌乳性狀的相關分析

鮑 斌,房興堂,陳 宏,張潤鋒,嚴林俊,張海軍   

  1. 徐州師范大學細胞與分子生物學研究所
  • 收稿日期:2006-10-31 修回日期:2007-03-12 出版日期:2008-06-10 發布日期:2008-06-10
  • 通訊作者: 陳宏

Polymorphism of Bovine STAT5A Gene and its Associations with Milk Performance Traits in Chinese Holstein Cattle

  

  1. 徐州師范大學細胞與分子生物學研究所
  • Received:2006-10-31 Revised:2007-03-12 Online:2008-06-10 Published:2008-06-10

摘要: 【目的】研究STAT5A基因多態性與中國荷斯坦牛泌乳性狀的相關性,為進一步加快育種進程提供參考依據。【方法】利用PCR-SSCP技術對279頭中國荷斯坦牛的STAT5A基因的P1和P2兩個位點進行群體遺傳學檢測;計算其基因頻率和基因型頻率,并對多態性與泌乳性狀進行相關性分析。【結果】發現在STAT5AP1和STAT5AP2兩個位點上分別存在3種多態以及7種單倍型組合。STAT5AP1位點的AA、GG和AG的基因型頻率分別為0.2401、0.1470和0.6129,A和G的基因頻率分別為0.5466和0.4534,不同基因型對第1胎產奶量、乳蛋白率和第2胎乳蛋白率存在顯著影響;STAT5AP2位點的CC、TT和CT的基因型頻率分別為0.7527、0.0036和0.2437,C和T的基因頻率分別為0.8746和0.1254,不同基因型對第2胎乳蛋白率有顯著影響;7種單倍型組合AACC、AGCC、AACT、AGCT、GGCT、GGCC和AATT的頻率分別為0.1864、0.4624、0.0502、0.1505、0.0430、0.1039和0.0036,不同單倍型與第1和2胎產奶量以及乳蛋白率存在相關性。【結論】STAT5A基因不同基因型和單倍型組合與中國荷斯坦牛產奶量和乳蛋白率存在相關性,但與乳脂率不相關。

關鍵詞: STAT5A, PCR-SSCP, 中國荷斯坦牛, 多態性, 單倍型

Abstract: PCR-SSCP was applied to analyze the polymorphisms of two locus in STAT5A gene in 279 Chinese Holstein cattle. Three genotypes were found at two locus and 7 haplotype combinations were present. The frequencies of genotype AA/BB/AB were 0.2401/0.1470/0.6129 and that of allele A/B in the population were 0.5466/0.4534 at locus P1. The result showed that the genotypes had significant effect on milk yield in the first lactations as also as protein rate in the second lactations. Genotype frequency of CC/DD/CD were 0.2401/0.1470/0.6129 and the frequencies of allele C/D in the population were 0.5466/0.4534 at locus P2. Different genotypes had remarkable influence on protein rate in the second lactations. In addition ,the frequencies of 7 haplotype combinations were 0.1864,0.4624,0.0502,0.1505,0.0430,0.1039 and 0.0036, different haplotype combinations affected milk yield, protein rate in the first and second lactations. No significant differences between individuals with different genotypes and haplotype combinations for milk fat rate in the first and second lactations at these two locus.

Key words: STAT5A, PCR-SSCP, Chinese Holstein cattle, Polymorphism, Haplotype

福利彩票3d开奖结果