中國農業科學 ?? 2008, Vol. 41 ?? Issue (7): 2092-2097 .doi: 10.3864/j.issn.0578-1752.2008.07.029

? 貯藏·保鮮·加工 ? 上一篇    下一篇

蕎麥籽粒乙醇提取物抗氧化成分的高效液相色譜電噴霧質譜研究

姚亞平,田呈瑞,曹 煒   

  1. 陜西師范大學食品工程與營養科學學院
  • 收稿日期:2007-08-12 修回日期:2007-10-29 出版日期:2008-07-10 發布日期:2008-07-10
  • 通訊作者: 田呈瑞

Studies on the Antioxidants of Buckwheat by HPLC/ ESI-MS

Ya-ping YAO   

  1. 陜西師范大學食品工程與營養科學學院
  • Received:2007-08-12 Revised:2007-10-29 Online:2008-07-10 Published:2008-07-10

摘要: 【目的】研究甜蕎麥和苦蕎麥籽粒乙醇提取物中的主要抗氧化成分。【方法】將蕎麥籽粒乙醇提取物與二苯基苦基苯肼(DPPH)自由基反應,然后用高效液相色譜儀識別乙醇提取物中的抗氧化成分,并用電噴霧質譜檢測器對抗氧化成分的結構信息進行表征,以確定蕎麥籽粒乙醇提取物中的抗氧成分。【結果】苦蕎麥和甜蕎麥籽粒乙醇提取物對DPPH自由基均有清除作用;高效液相色譜分析表明,甜蕎麥和苦蕎麥乙醇提取物中均含有兩種主要抗氧化峰,且對應的色譜保留時間和光譜信息相同;電噴霧質譜分析表明,蕎麥籽粒乙醇提取物中抗氧化成分的分子量和質譜碎裂行為分別與對照品蘆丁和槲皮素的相同。【結論】采用HPLC-MS/MS法可以快速篩選蕎麥籽粒提取物中的抗氧化成分,蕎麥籽粒提取物中的抗氧化成分主要為蘆丁和槲皮素,其中槲皮素的抗氧化活性高于蘆丁。

關鍵詞: 蕎麥, 抗氧化成分, HPLC, MS/MS

Abstract: [Objective] To study the main antioxidants in common buckwheat and tartary buckwheat . [Method] It takes the buckwheat ethanol extraction subtracts with the DPPH free radicals reaction, identify the antioxidants in the extrcats with HPLC, study the structure character of antioxidation compositions by Electro Spray Ionization-Mass Spectrometry (ESI-MS), and determine the antioxidation compositions of buckwheat ethanol extracts. [Result] Both the common buckwheat and the tartary buckwheat ethanol extracts can scavenge free radical. From the HPLC figure there were two main peaks, which included two kinds of antioxidants in the extracts. Meanwhile, the corresponding chromatograph retention time was the same as the spectrum information. The ESI-MS indicated that, the molecular weights and MS fragmentation pattern of antioxidants in the extracts were similar to the reference material of rutin and quercetin. [Conclusion] The method of HPLC-MS/MS can be used to screen the antioxidants in the extraction quickly; the main antioxidants of the buckwheat extracts were rutin and quercetin, while the activity of antioxidant activity of quercetin was higher than rutin.

Key words: Buckwheat, Antioxidant, HPLC, MS/MS

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