中國農業科學 ?? 2013, Vol. 46 ?? Issue (17): 3721-3728.doi: 10.3864/j.issn.0578-1752.2013.17.022

? 畜牧·獸醫·資源昆蟲 ? 上一篇    下一篇

婚飛對中華蜜蜂性成熟處女蜂王sRNAs表達的影響

 吳小波, 王子龍, 石元元, 張飛, 曾志將   

  1. 江西農業大學蜜蜂研究所,南昌 330045
  • 收稿日期:2013-02-06 出版日期:2013-09-01 發布日期:2013-04-11
  • 通訊作者: 通信作者曾志將,E-mail:[email protected] E-mail:[email protected]
  • 作者簡介:吳小波,E-mail:[email protected]
  • 基金資助:

    國家自然科學基金項目(31060327)、江西農業大學青年科學基金項目(2010-09003303)、江西省研究生創新基金項目(YC2011-B016)

Effects of Mating Flight on sRNAs Expression in Sexual Matured Virgin Queens (Apis cerana cerana)

 WU  Xiao-Bo, WANG  Zi-Long, SHI  Yuan-Yuan, ZHANG  Fei, ZENG  Zhi-Jiang   

  1. Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045
  • Received:2013-02-06 Online:2013-09-01 Published:2013-04-11

摘要: 【目的】研究中華蜜蜂(Apis cerana cerana)性成熟處女蜂王在婚飛過程中sRNAs表達變化。【方法】以中華蜜蜂性成熟處女蜂王為試驗材料,通過控制部分蜂王在一定區域飛行,并利用高通量測序的方法檢測并分析中華蜜蜂性成熟處女蜂王飛行和未飛行之間的sRNAs表達差異。【結果】飛行蜂王和未飛行蜂王均含有豐富的sRNAs,主要分布在22 nt和27—29 nt,但各類sRNA在2種狀態的蜂王中分布比例不同;2個文庫共有總序列數占2個樣品總序列數的92.79%,而且飛行蜂王中的sRNAs種類比未飛行蜂王多;與已知miRNA比對分析,發現飛行蜂王和未飛行蜂王有25個極顯著差異表達miRNA,其中只有ame-miR-750在飛行蜂王中上調,其它24個極顯著差異表達miRNA在飛行蜂王中下調;有19個差異表達miRNA所對應的11個靶基因在飛行蜂王和未飛行蜂王之間存在表達差異。【結論】中華蜜蜂性成熟處女蜂王在婚飛過程中,體內大量sRNAs表達發生了變化,可能為性成熟處女蜂王正常交配起著重要的生理調節作用。

關鍵詞: 中華蜜蜂 , 蜂王 , 飛行 , miRNA

Abstract: 【Objective】The objective of this study is to analyze the influence of flight behaviour on sRNAs expression in matured virgin queens (Apis cerana cerana). 【Method】The sexual matured virgin queens of A. cerana cerana were used as flying group that allow to fly in a certain area and the other group was not. The sRNAs expression differences between the two groups were analyzed by using a high-throughput sequencing method. 【Result】Both of the flying matured virgin queens and non-flying counterparts had a complicated sRNAs population, and that the length of sRNAs varied, 22 nt and 27-29 nt being the predominant length, while the frequence percent of the same RNA was different in the two samples. Combining deep sequencing and bioinformatics analysis, the percent of the common sequences in total sRNAs was 92.79%, but the number of unique sRNAs in the flying queens was more than that in non-flying queens. Matching to known miRNA alignment, a total of 25 known miRNAs were significantly different between them, with 1 up-regulated and 24 down-regulated in the flying matured virgin queens while there were 11 target genes of 19 known miRNA which were expressed differentially between them.【Conclusion】There are a large number of sRNAs changes during the flight process of matured virgin queens which may play an important role in adjusting physiology for mating successfully of virgin queens.

Key words: Apis cerana cerana , queen bee , flight , miRNA

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