中國農業科學 ?? 2019, Vol. 52 ?? Issue (17): 2921-2928.doi: 10.3864/j.issn.0578-1752.2019.17.001

? 作物遺傳育種·種質資源·分子遺傳學 ? 上一篇    下一篇

稻米抗性淀粉含量及其環境穩定性分析

張春龍,Channarong PHONGSAI,張江麗,于洋,蘇耀華,楊米,高亮,普世皇,李娟,金壽林,譚學林,文建成()   

  1. 云南農業大學稻作研究所,昆明 650201
  • 收稿日期:2019-02-27 接受日期:2019-05-27 出版日期:2019-09-01 發布日期:2019-09-10
  • 通訊作者: 文建成 E-mail:[email protected]
  • 作者簡介:張春龍,E-mail:[email protected]。|Phongsai Channarong,E-mail:[email protected]
  • 基金資助:
    云南省重點新產品開發計劃(2015BB015);云南省院士工作站項目(2018IC065)

Evaluation of Rice Germplasms for Grain Resistant Starch Content and Its Environmental Stability

ZHANG ChunLong,Channarong PHONGSAI,ZHANG JiangLi,YUYang,SU YaoHua,YANG Mi,GAO Liang,PU ShiHuang,LI Juan,JIN ShouLin,TAN XueLin,WEN JianCheng()   

  1. Rice Research Institute of Yunnan Agricultural University, Kunming 650201
  • Received:2019-02-27 Accepted:2019-05-27 Online:2019-09-01 Published:2019-09-10
  • Contact: WEN JianCheng E-mail:[email protected]

摘要:

【目的】食用高抗性淀粉含量稻米雖利于提高慢性病人群的健康水平,但培育出的高抗性淀粉含量水稻品種還較少。開展水稻種質資源抗性淀粉含量及其環境穩定性的研究,為高抗性淀粉含量水稻種質資源發掘和生產應用提供參考依據。【方法】參照愛爾蘭Megazyme公司提供的方法測定稻米抗性淀粉含量,用PAST軟件完成種質含量分布作圖。通過一年多點試驗評價抗性淀粉含量的環境穩定性,利用DPS軟件完成含量方差分析。依據國家標準GB/T15683-2008分析了稻米直鏈淀粉含量。【結果】對1 206份水稻種質稻米抗性淀粉含量分析,結果表明,絕大部分水稻種質稻米抗性淀粉含量低,含量低于2.5%的占87.6%,高于10%的僅占約0.2%。稻米抗性淀粉和直鏈淀粉含量存在顯著正相關,但在高直鏈淀粉含量種質中未出現抗性淀粉含量高的品系,卻在低直鏈淀粉含量種質中發現3份抗性淀粉含量高于10%的品系,其中1個優質軟米品種Diangu2的抗性淀粉和直鏈淀粉含量分別為10.12%和12.3%,綜合農藝性狀優良,米飯食味性好。在3個不同環境種植18個不同抗性淀粉含量的品系,結果顯示有13個品系的含量不受種植環境差異的影響,另5個品系的含量受環境影響,稻米的抗性淀粉含量除了受基因型影響外,還受種植環境、以及基因型與環境互作的影響。【結論】水稻種質資源稻米抗性淀粉含量普遍很低,抗性淀粉含量與直鏈淀粉含量雖存在顯著正相關,但低直鏈淀粉含量種質中也可能存在高抗性淀粉的品種,所以培育稻米抗性淀粉含量高且食口性好的品種是可能的。稻米抗性淀粉含量主要受基因型控制,在不同環境中含量高的品種依然高,含量低的品種仍然低,那么高含量的品種可在其適種稻區種植生產大米,其含量不會被明顯影響。

關鍵詞: 水稻, 種質資源, 抗性淀粉, 直鏈淀粉, 環境穩定性

Abstract:

【Objective】 It is beneficial for improving the health of chronic patients to eat high resistant starch (RS) content rice, but variety with high grain RS content is still very rare. Evaluations of rice germplasms for grain RS contents and its environmental stability were carried out in this study, and the results could provide a reference for the discovery of rice germplasm resources with high RS contents and its production. 【Method】 Grain RS contents in rice germplasms were detected by the Megazyme method, and used the PAST software to complete the frequency distribution of these contents. The environmental stability of rice RS contents were tested by planting at multiple locations within one year, and its variance analysis were performed with DPS software. Grain amylase contents were tested according to the national standard GB/T15683-2008. 【Result】 The initial evaluations on RS contents of 1206 rice germplasms demonstrated that for most these rice with RS content were very low, and about 87.6% of the rice were less than 2.5%, only about 0.2% were higher than 10%. There was a significant positive correlation between rice RS and amylose content, but high RS variety was not discovered in high amylose germplasms. However, three varieties with RS content higher than 10% were screened in low amylose content germplasms, and one of them was a good quality soft rice variety Diangu2, which has RS and amylose content of 10.12% and 12.3%, respectively. It carried out regional tests for eighteen varieties with different RS contents planting at three very differ environmental conditions. The result of environmental stability analysis indicated that the contents of thirteen varieties were affected by differences in planting area, of the others were affected. Totally, the contents were affected by genotype, planting environment and genotype-by-environment interactions. 【Conclusion】 In this research, most rice resources have low RS content, and also detected a significant positive correlation between rice grain RS and amylase content. Therefore, it is possible to develop rice variety with high RS starch and good taste, according the high RS varieties were identified in low amylose content germplasms. The RS content was mainly affected by genotype, so the varieties with high RS contents were high at different environments, and the lows’ were still low. Then, the varieties with high RS content can grow in its suitable areas to produce rice, and the contents could not be significantly affected.

Key words: rice (Oryza sativa L.), germplasm resource, resistant starch, amylose, environmental stability

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